A radical enzyme intermediate trapped using non-native substrates

Fe–S centers in the HydG X-ray crystal structure
Fe–S centers in the HydG X-ray crystal structure

In a recent publication "Trapping and Electron Paramagnetic Resonance Characterization of the 5′dAdo• Radical in a Radical S-Adenosyl Methionine Enzyme Reaction with a Non-Native Substrate", Rick Sayler and coworkers in R. David Britt's Lab at UC Davis used non-natural substrates in the HydG radical SAM enzyme to trap and characterize the hot 5'deoxyadenosyl radical for the first time in such an enzyme reaction.

More information at https://pubs.acs.org/doi/10.1021/acscentsci.9b00706